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Single molecule kinetics of horseradish peroxidase exposed in large arrays of femtoliter-sized fused silica chambers

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Ehrl, Benno ; Liebherr, Raphaela ; Gorris, H. H.
License: Allianz- bzw. Nationallizenz
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Date of publication of this fulltext: 04 Aug 2016 08:31


Large arrays of femtoliter-sized chambers were etched into the surface of fused silica slides to enclose and observe hundreds of single horseradish peroxidase (HRP) molecules in parallel. Individual molecules of HRP oxidize the fluorogenic substrate Amplex Red to fluorescent resorufin in separate chambers, which was monitored by fluorescence microscopy. Photooxidation of Amplex Red and ...


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