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Single molecule kinetics of horseradish peroxidase exposed in large arrays of femtoliter-sized fused silica chambers

Ehrl, Benno, Liebherr, Raphaela and Gorris, H.H. (2013) Single molecule kinetics of horseradish peroxidase exposed in large arrays of femtoliter-sized fused silica chambers. Analyst 138, 4260- 4265.

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Abstract

Large arrays of femtoliter-sized chambers were etched into the surface of fused silica slides to enclose and observe hundreds of single horseradish peroxidase (HRP) molecules in parallel. Individual molecules of HRP oxidize the fluorogenic substrate Amplex Red to fluorescent resorufin in separate chambers, which was monitored by fluorescence microscopy. Photooxidation of Amplex Red and ...

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Item type:Article
Date:2013
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Identification Number:
ValueType
10.1039/C3AN00809FDOI
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:34240
Owner only: item control page

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