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Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis

Kirschneck, Christian , Batschkus, Sarah, Proff, Peter, Köstler, Josef, Spanier, Gerrit and Schröder, Agnes (2017) Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis. Scientific Reports 2017 (7), pp. 1-13.

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Date of publication of this fulltext: 22 Jan 2018 11:26

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Other URL: https://www.nature.com/articles/s41598-017-15281-0

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Abstract

Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression ...

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Item type:Article
Date:7 November 2017
Institutions:Medicine > Lehrstuhl für Kieferorthopädie
Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.1038/s41598-017-15281-0DOI
Article number: 14751Other
Keywords:REAL-TIME PCR; LIGAMENT CELLS; QUANTITATIVE PCR; MINIMUM INFORMATION; HOUSEKEEPING GENES; UP-REGULATION; IN-VITRO; HYPOXIA; RNA; IDENTIFICATION;
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:36212
Owner only: item control page

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