Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis

URN to cite this document:: urn:nbn:de:bvb:355-epub-362120
DOI to cite this document:: 10.5283/epub.36212

Kirschneck, Christian

; Batschkus, Sarah ; Proff, Peter ; Köstler, Josef ; Spanier, Gerrit ; Schröder, Agnes

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Date of publication of this fulltext: 22 Jan 2018 11:26

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Abstract

Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression ...