Kirschneck, Christian 
, Batschkus, Sarah, Proff, Peter, Köstler, Josef, Spanier, Gerrit and Schröder, Agnes
(2017)
Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis.
Scientific Reports 2017 (7), pp. 1-13.
| License: Creative Commons Attribution 4.0 PDF - Submitted Version Download (3MB) |
Other URL: https://www.nature.com/articles/s41598-017-15281-0
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Abstract
Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression ...
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| Item type: | Article | ||||||
|---|---|---|---|---|---|---|---|
| Date: | 7 November 2017 | ||||||
| Institutions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie | ||||||
| Projects: | Open Access Publizieren (DFG) | ||||||
| Identification Number: |
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| Keywords: | REAL-TIME PCR; LIGAMENT CELLS; QUANTITATIVE PCR; MINIMUM INFORMATION; HOUSEKEEPING GENES; UP-REGULATION; IN-VITRO; HYPOXIA; RNA; IDENTIFICATION; | ||||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||||
| Status: | Published | ||||||
| Refereed: | Yes, this version has been refereed | ||||||
| Created at the University of Regensburg: | Yes | ||||||
| Item ID: | 36212 |
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