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A highly efficient strategy to determine genotypes of genetically-engineered mice using genomic DNA purified from hair roots

Otaño-Rivera, Víctor, Boakye, Amma, Grobe, Nadja, Almutairi, Mohammed M, Kursan, Shams, Mattis, Lesan K, Castrop, Hayo, Gurley, Susan B, Elased, Khalid M, Boivin, Gregory P and Di Fulvio, Mauricio (2017) A highly efficient strategy to determine genotypes of genetically-engineered mice using genomic DNA purified from hair roots. Laboratory Animals 51 (2), pp. 138-146.

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Other URL: http://doi.org/10.1177/0023677216646088


Abstract

Genotyping of genetically-engineered mice is necessary for the effective design of breeding strategies and identification of mutant mice. This process relies on the identification of DNA markers introduced into genomic sequences of mice, a task usually performed using the polymerase chain reaction (PCR). Clearly, the limiting step in genotyping is isolating pure genomic DNA. Isolation of mouse ...

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Item type:Article
Date:2017
Institutions:Biology, Preclinical Medicine > Institut für Physiologie
Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop
Identification Number:
ValueType
10.1177/0023677216646088DOI
Keywords:EXTRACTION PROTOCOL; TRANSGENIC MICE; BLOOD-PRESSURE; TAIL BIOPSY; PCR; IDENTIFICATION; RESPONSES; CHELEX; TISSUE; MOUSE; 3Rs; alternatives; welfare; rodents; DNA isolation
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:39111
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