Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

URN to cite this document:: urn:nbn:de:bvb:355-epub-407169
DOI to cite this document:: 10.5283/epub.40716

Penzkofer, Alfons ; Silapetere, Arita ; Hegemann, Peter

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Date of publication of this fulltext: 18 Sep 2019 09:59

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Abstract

QuasAr1 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense by directed evolution. Here we report absorption and emission spectroscopic studies of QuasAr1 in Tris buffer at pH 8. Absorption cross-section spectra, fluorescence quantum distributions, fluorescence quantum yields, and fluorescence excitation spectra were determined. The thermal stability of ...