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Sterility testing of blood stem cell products is possible in the presence of 1 ml of a DMSO containing stem cell product

Richter, E. ; Tapernon, K. ; Friedrich, A. W. ; Sibrowski, W. ; Schmitz, Gerd ; Ahrens, Norbert


Background: Bacterial contamination of stem cell products may causefatal clinical consequences in immunosuppressed patients. Sterility testingof blood stem cell products is often done with blood culture bottles prior tocryopreservation. It is not known, if cell suspension components as e.g.DMSO impair the assay sensitivity and if an aliquot of 1 ml is great enoughfor the reliable ...


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