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Journal or Publication Title: | Nature | ||||
Publisher: | NATURE PUBLISHING GROUP | ||||
Place of Publication: | LONDON | ||||
Volume: | 507 | ||||
Number of Issue or Book Chapter: | 7493 | ||||
Page Range: | pp. 455-461 | ||||
Date: | 2014 | ||||
Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Leibniz Institute for Immunotherapy (LIT) | ||||
Identification Number: |
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Keywords: | TRANSCRIPTION FACTORS; GENE-EXPRESSION; CAP-ANALYSIS; REGULATORY SEQUENCES; DNA METHYLATION; DISCOVERY; INFORMATION; LANDSCAPE; UPSTREAM; PATTERN; | ||||
Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
Status: | Published | ||||
Refereed: | Yes, this version has been refereed | ||||
Created at the University of Regensburg: | Yes | ||||
Item ID: | 61633 |
Abstract
Enhancers control the correct temporal and cell-type-specific activation of gene expression in multicellular eukaryotes. Knowing their properties, regulatory activity and targets is crucial to understand the regulation of differentiation and homeostasis. Here we use the FANTOM5 panel of samples, covering the majority of human tissues and cell types, to produce an atlas of active, in ...

Abstract
Enhancers control the correct temporal and cell-type-specific activation of gene expression in multicellular eukaryotes. Knowing their properties, regulatory activity and targets is crucial to understand the regulation of differentiation and homeostasis. Here we use the FANTOM5 panel of samples, covering the majority of human tissues and cell types, to produce an atlas of active, in vivo-transcribed enhancers. We show that enhancers share properties with CpG-poor messenger RNA promoters but produce bidirectional, exosome-sensitive, relatively short unspliced RNAs, the generation of which is strongly related to enhancer activity. The atlas is used to compare regulatory programs between different cells at unprecedented depth, to identify disease-associated regulatory single nucleotide polymorphisms, and to classify cell-type-specific and ubiquitous enhancers. We further explore the utility of enhancer redundancy, which explains gene expression strength rather than expression patterns. The online FANTOM5 enhancer atlas represents a unique resource for studies on cell-type-specific enhancers and gene regulation.
Metadata last modified: 19 Dec 2024 08:12