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Detection of influenza A(H1N1)v virus by real-time RT-PCR.

URN to cite this document:
urn:nbn:de:bvb:355-epub-99956
Panning, M. ; Eickmann, M. ; Landt, O. ; Monazahian, M. ; Olschläger, S. ; Baumgarte, S. ; Reischl, U. ; Wenzel, J. J. ; Niller, H. H. ; Günther, S. ; Hollmann, B. ; Huzly, D. ; Drexler, J. F. ; Helmer, A. ; Becker, S. ; Matz, B. ; Eis-Hübinger, Am. ; Drosten, C.
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Date of publication of this fulltext: 28 Oct 2009 08:26


Abstract

Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference ...

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