Detection of influenza A(H1N1)v virus by real-time RT-PCR.

• URN zum Zitieren dieses Dokuments: urn:nbn:de:bvb:355-epub-99956

Panning, M., Eickmann, M., Landt, O., Monazahian, M., Olschläger, S., Baumgarte, S., Reischl, U., Wenzel, J. J., Niller, H. H., Günther, S., Hollmann, B., Huzly, D., Drexler, J. F., Helmer, A., Becker, S., Matz, B., Eis-Hübinger, Am. und Drosten, C. (2009) Detection of influenza A(H1N1)v virus by real-time RT-PCR. Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin 14 (36).

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Zusammenfassung

Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference ...

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