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Tris: an allosteric effector of tarantula haemocyanin.

Sterner, Reinhard ; Bardehle, K. ; Paul, R. ; Decker, H.



Abstract

The effect of the chemical buffering component Tris (hydroxy-methyl-amino-methane) and of chloride ions on the oxygen binding of tarantula hemocyanin was studied at constant pH. It revealed that Tris at micromolar concentrations decreases the oxygen pressure at half-saturation (p50) by a factor of more than two, whereas chloride does not influence oxygen affinity. A thermodynamic analysis in ...

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