A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail

URN to cite this document:: urn:nbn:de:bvb:355-epub-159001
DOI to cite this document:: 10.5283/epub.15900

Witzgall, Ralph ; O'Leary, Eileen ; Bonventre, Joseph V.

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Date of publication of this fulltext: 06 Dec 2010 09:14

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Abstract

Expression of newly cloned cDNAs in mammalian cell lines is an essential tool for the functional analysis of the proteins encoded by these cDNAs. In many instances, however, evaluation of the protein is difficult because of the difficulty in purification of the expressed protein and/or the lack of specific antibodies which react with the proteins on Western blots or for immunocytochemistry or ...