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Flauger, Birgit ; Krueger, Konstanze ; Gerhards, Hartmut ; Möstl, Erich

Simplified method to measure glucocorticoid metabolites in faeces of horses

Flauger, Birgit, Krueger, Konstanze , Gerhards, Hartmut und Möstl, Erich (2010) Simplified method to measure glucocorticoid metabolites in faeces of horses. Veterinary research communications 34 (2), S. 185-195.

Veröffentlichungsdatum dieses Volltextes: 19 Aug 2011 07:25
Artikel
DOI zum Zitieren dieses Dokuments: 10.5283/epub.21815


Zusammenfassung

Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited practicability ...

Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited practicability due to its complex extraction procedure. Therefore, we tested the applicability of other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoaetiocholanolone using 11-oxoaetiocholanolone-17-CMO: BSA (3 alpha,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3 alpha,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test, lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species.



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Details

DokumentenartArtikel
Titel eines Journals oder einer ZeitschriftVeterinary research communications
Verlag:SPRINGER
Ort der Veröffentlichung:DORDRECHT
Band:34
Nummer des Zeitschriftenheftes oder des Kapitels:2
Seitenbereich:S. 185-195
Datum2010
InstitutionenBiologie und Vorklinische Medizin > Institut für Zoologie > Zoologie/Evolutionsbiologie (Prof. Dr. Jürgen Heinze)
Identifikationsnummer
WertTyp
20182914PubMed-ID
10.1007/s11259-010-9344-yDOI
Klassifikation
NotationArt
Adrenocorticotropic Hormone/pharmacologyMESH
AnimalsMESH
Dexamethasone/metabolismMESH
Etiocholanolone/metabolismMESH
Feces/chemistryMESH
FemaleMESH
Glucocorticoids/metabolismMESH
Horses/metabolismMESH
Immunoenzyme Techniques/veterinaryMESH
MaleMESH
Stress, Physiological/physiologyMESH
Stichwörter / KeywordsFECAL CORTISOL METABOLITES; DOMESTIC LIVESTOCK; STRESS; VALIDATION; HORMONES; ACTH challenge; Enzyme immunoassay; Stress behaviour; Cortisol
Dewey-Dezimal-Klassifikation500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
StatusVeröffentlicht
BegutachtetJa, diese Version wurde begutachtet
An der Universität Regensburg entstandenJa
URN der UB Regensburgurn:nbn:de:bvb:355-epub-218151
Dokumenten-ID21815

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