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Flauger, Birgit ; Krueger, Konstanze ; Gerhards, Hartmut ; Möstl, Erich

Simplified method to measure glucocorticoid metabolites in faeces of horses

Flauger, Birgit, Krueger, Konstanze , Gerhards, Hartmut and Möstl, Erich (2010) Simplified method to measure glucocorticoid metabolites in faeces of horses. Veterinary research communications 34 (2), pp. 185-195.

Date of publication of this fulltext: 19 Aug 2011 07:25
Article
DOI to cite this document: 10.5283/epub.21815


Abstract

Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited practicability ...

Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited practicability due to its complex extraction procedure. Therefore, we tested the applicability of other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoaetiocholanolone using 11-oxoaetiocholanolone-17-CMO: BSA (3 alpha,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3 alpha,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test, lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species.



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Details

Item typeArticle
Journal or Publication TitleVeterinary research communications
Publisher:SPRINGER
Place of Publication:DORDRECHT
Volume:34
Number of Issue or Book Chapter:2
Page Range:pp. 185-195
Date2010
InstitutionsBiology, Preclinical Medicine > Institut für Zoologie > Zoologie/Evolutionsbiologie (Prof. Dr. Jürgen Heinze)
Identification Number
ValueType
20182914PubMed ID
10.1007/s11259-010-9344-yDOI
Classification
NotationType
Adrenocorticotropic Hormone/pharmacologyMESH
AnimalsMESH
Dexamethasone/metabolismMESH
Etiocholanolone/metabolismMESH
Feces/chemistryMESH
FemaleMESH
Glucocorticoids/metabolismMESH
Horses/metabolismMESH
Immunoenzyme Techniques/veterinaryMESH
MaleMESH
Stress, Physiological/physiologyMESH
KeywordsFECAL CORTISOL METABOLITES; DOMESTIC LIVESTOCK; STRESS; VALIDATION; HORMONES; ACTH challenge; Enzyme immunoassay; Stress behaviour; Cortisol
Dewey Decimal Classification500 Science > 590 Zoological sciences
500 Science > 590 Zoological sciences
StatusPublished
RefereedYes, this version has been refereed
Created at the University of RegensburgYes
URN of the UB Regensburgurn:nbn:de:bvb:355-epub-218151
Item ID21815

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