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Long time monitoring of the respiratory activity of isolated mitochondria

Heller, Anne ; Fischer, Lorenz H. ; Wolfbeis, Otto S. ; Göpferich, Achim



Abstract

We have investigated the ability of optical oxygen sensors incorporated in a microplate to determine the respiratory activity of cell fractions. Different cell fractions were monitored, in particular to evaluate the long term functionality of isolated mitochondria. It is possible to continuously sense respiratory activity of isolated mitochondria over time. We found that they are functional for ...

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