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Local Tertiary Structure Probing of Ribonucleoprotein Particles by Nuclease Fusion Proteins

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Ohmayer, Uli ; Perez-Fernandez, Jorge ; Hierlmeier, Thomas ; Pöll, Gisela ; Williams, Lydia ; Griesenbeck, Joachim ; Tschochner, Herbert ; Milkereit, Philipp
License: Creative Commons: Attribution 3.0
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Date of publication of this fulltext: 07 Sep 2012 06:58


Analyses of the conformational dynamics of the numerous cellular ribonucleoprotein particles (RNP) significantly contribute to the understanding of their modes of action. Here, we tested whether ribonuclease fusion proteins incorporated into RNPs can be used as molecular probes to characterize the local RNA environment of these proteins. Fusion proteins of micrococcal nuclease (MNase) with ...


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