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siPools: highly complex but accurately defined siRNA pools eliminate off-target effects

Hannus, Michael, Beitzinger, Michaela, Engelmann, Julia C., Weickert, Marie-Theresa, Spang, Rainer, Hannus, Stefan and Meister, Gunter (2014) siPools: highly complex but accurately defined siRNA pools eliminate off-target effects. Nucleic Acids Research 42, pp. 8049-8061.

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Date of publication of this fulltext: 30 Jul 2014 11:42

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Abstract

Short interfering RNAs (siRNAs) are widely used as tool for gene inactivation in basic research and therapeutic applications. One of the major shortcomings of siRNA experiments are sequence-specific off-target effects. Such effects are largely unpredictable because siRNAs can affect partially complementary sequences and function like microRNAs (miRNAs), which inhibit gene expression on mRNA ...

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Item type:Article
Date:29 May 2014
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang)
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.1093/nar/gku480DOI
24875475PubMed ID
Keywords:HeLa Cells; Humans; Interferons, pharmacology; Multigene Family; RNA Interference; RNA, Messenger, metabolism; RNA, Small Interfering, biosynthesis/metabolism
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:30494
Owner only: item control page

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