siPools: highly complex but accurately defined siRNA pools eliminate off-target effects

URN to cite this document:: urn:nbn:de:bvb:355-epub-304949
DOI to cite this document:: 10.5283/epub.30494

Hannus, Michael ; Beitzinger, Michaela ; Engelmann, Julia C.

; Weickert, Marie-Theresa ; Spang, Rainer ; Hannus, Stefan ; Meister, Gunter

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Date of publication of this fulltext: 30 Jul 2014 11:42

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Abstract

Short interfering RNAs (siRNAs) are widely used as tool for gene inactivation in basic research and therapeutic applications. One of the major shortcomings of siRNA experiments are sequence-specific off-target effects. Such effects are largely unpredictable because siRNAs can affect partially complementary sequences and function like microRNAs (miRNAs), which inhibit gene expression on mRNA ...