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Raf kinases mediate the phosphorylation of eukaryotic translation elongation factor 1A and regulate its stability in eukaryotic cells

Sanges, C., Scheuermann, C., Zahedi, R. P., Sickmann, A., Lamberti, A., Migliaccio, N., Baljuls, A., Marra, M., Zappavigna, S., Reinders, Jörg, Rapp, U., Abbruzzese, A., Caraglia, M. and Arcari, P. (2012) Raf kinases mediate the phosphorylation of eukaryotic translation elongation factor 1A and regulate its stability in eukaryotic cells. Cell Death and Disease 3, e276.

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Abstract

We identified eukaryotic translation elongation factor 1A (eEF1A) Raf-mediated phosphorylation sites and defined their role in the regulation of eEF1A half-life and of apoptosis of human cancer cells. Mass spectrometry identified in vitro S21 and T88 as phosphorylation sites mediated by B-Raf but not C-Raf on eEF1A1 whereas S21 was phosphorylated on eEF1A2 by both B- and C-Raf. Interestingly, S21 ...

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Item type:Article
Date:March 2012
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
22378069PubMed ID
10.1038/cddis.2012.16DOI
Classification:
NotationType
AnimalsMESH
Apoptosis/geneticsMESH
COS CellsMESH
Cercopithecus aethiopsMESH
Gene Expression RegulationMESH
HumansMESH
Models, MolecularMESH
MutationMESH
Peptide Elongation Factor 1/metabolismMESH
Phosphoproteins/metabolismMESH
PhosphorylationMESH
Protein BindingMESH
Protein MultimerizationMESH
Protein StabilityMESH
Protein Structure, TertiaryMESH
Proto-Oncogene Proteins B-raf/metabolismMESH
Proto-Oncogene Proteins c-raf/metabolismMESH
Recombinant Proteins/metabolismMESH
Signal TransductionMESH
TransfectionMESH
Keywords:EF-1A; Raf kinases; signal transduction; apoptosis; ubiquitin; mass spectrometry
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:30574
Owner only: item control page

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