Lyck, Ruth, Ruderisch, Nadine, Moll, Anton G., Steiner, Oliver, Cohen, Clemens D., Engelhardt, Britta, Makrides, Victoria und Verrey, Francois
(2009)
Culture-induced changes in blood-brain barrier transcriptome: implications for amino-acid transporters in vivo.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 29 (9), S. 1491-1502.
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Zusammenfassung
Tight homeostatic control of brain amino acids (AA) depends on transport by solute carrier family proteins expressed by the blood-brain barrier (BBB) microvascular endothelial cells (BMEC). To characterize the mouse BMEC transcriptome and probe culture-induced changes, microarray analyses of platelet endothelial cell adhesion molecule-1-positive (PECAM1(+)) endothelial cells (ppMBMECs) were ...
Zusammenfassung
Tight homeostatic control of brain amino acids (AA) depends on transport by solute carrier family proteins expressed by the blood-brain barrier (BBB) microvascular endothelial cells (BMEC). To characterize the mouse BMEC transcriptome and probe culture-induced changes, microarray analyses of platelet endothelial cell adhesion molecule-1-positive (PECAM1(+)) endothelial cells (ppMBMECs) were compared with primary MBMECs (pMBMEC) cultured in the presence or absence of glial cells and with b.End5 endothelioma cell line. Selected cell marker and AA transporter mRNA levels were further verified by reverse transcription real-time PCR. Regardless of glial coculture, expression of a large subset of genes was strongly altered by a brief culture step. This is consistent with the known dependence of BMECs on in vivo interactions to maintain physiologic functions, for example, tight barrier formation, and their consequent dedifferentiation in culture. Seven (4F2hc, Lat1, Taut, Snat3, Snat5, Xpct, and Cat1) of nine AA transporter mRNAs highly expressed in freshly isolated ppMBMECs were strongly downregulated for all cultures and two (Snat2 and Eaat3) were variably regulated. In contrast, five AA transporter mRNAs with low expression in ppMBMECs, including y(+)Lat2, xCT, and Snat1, were upregulated by culture. We hypothesized that the AA transporters highly expressed in ppMBMECs and downregulated in culture have a major in vivo function for BBB transendothelial transport.
Bibliographische Daten exportieren
| Dokumentenart: | Artikel |
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| Datum: | September 2009 |
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| Institutionen: | Medizin > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) |
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| Identifikationsnummer: | | Wert | Typ |
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| 19491922 | PubMed-ID | | 10.1038/jcbfm.2009.72 | DOI |
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| Klassifikation: | | Notation | Art |
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| Amino Acid Transport Systems/metabolism | MESH | | Animals | MESH | | Antigens, CD31/metabolism | MESH | | Biological Markers/metabolism | MESH | | Blood-Brain Barrier/physiology | MESH | | Cell Culture Techniques | MESH | | Cells, Cultured | MESH | | Cerebrovascular Circulation | MESH | | Endothelial Cells/physiology | MESH | | Female | MESH | | Gene Expression Profiling | MESH | | Homeostasis | MESH | | Mice | MESH | | Mice, Inbred C57BL | MESH | | Microarray Analysis | MESH | | Microcirculation | MESH | | Molecular Sequence Data | MESH | | Neuroglia/metabolism | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Nein |
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| Eingebracht am: | 19 Aug 2014 07:41 |
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| Zuletzt geändert: | 18 Jan 2016 12:45 |
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| Dokumenten-ID: | 30671 |
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