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Culture-induced changes in blood-brain barrier transcriptome: implications for amino-acid transporters in vivo

Lyck, Ruth ; Ruderisch, Nadine ; Moll, Anton G. ; Steiner, Oliver ; Cohen, Clemens D. ; Engelhardt, Britta ; Makrides, Victoria ; Verrey, Francois



Abstract

Tight homeostatic control of brain amino acids (AA) depends on transport by solute carrier family proteins expressed by the blood-brain barrier (BBB) microvascular endothelial cells (BMEC). To characterize the mouse BMEC transcriptome and probe culture-induced changes, microarray analyses of platelet endothelial cell adhesion molecule-1-positive (PECAM1(+)) endothelial cells (ppMBMECs) were ...

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