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Detecting inactivated endospores in fluorescence microscopy using propidium monoazide

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Probst, Alexander ; Mahnert, A. ; Weber, Christina ; Haberer, Klaus ; Moissl-Eichinger, Christine
License: Allianz- bzw. Nationallizenz
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Date of publication of this fulltext: 13 Oct 2016 09:37


The differentiation between living and dead bacterial endospores is crucial in many research areas of microbiology. The identification of inactivated, non-pathogenic Bacillus anthracis spores is one reason why improvement of decontamination protocols is so desirable. Another field interested in spore viability is planetary protection, a sub-discipline of astrobiology that estimates the bioburden ...


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