The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences

URN to cite this document:: urn:nbn:de:bvb:355-epub-361409
DOI to cite this document:: 10.5283/epub.36140

Längst, Gernot ; König, Fabian ; Schubert, Thomas E. O.

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Date of publication of this fulltext: 04 Sep 2017 09:55

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Abstract

The monoclonal antibody S9.6 is a widely-used tool to purify, analyse and quantify R-loop structures in cells. A previous study using the surface plasmon resonance technology and a single-chain variable fragment (scFv) of S9.6 showed high affinity (0.6 nM) for DNA-RNA and also a high affinity (2.7 nM) for RNA-RNA hybrids. We used the microscale thermo-phoresis method allowing surface independent ...