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The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences

Längst, Gernot, König, Fabian and Schubert, Thomas E. O. (2017) The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PLoS ONE 12 (6), e0178875.

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Date of publication of this fulltext: 04 Sep 2017 09:55

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Other URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178875

Dieser Artikel ist in einer Zeitschrift aus dem Directory of Open Access (DOAJ) publiziert.


Abstract

The monoclonal antibody S9.6 is a widely-used tool to purify, analyse and quantify R-loop structures in cells. A previous study using the surface plasmon resonance technology and a single-chain variable fragment (scFv) of S9.6 showed high affinity (0.6 nM) for DNA-RNA and also a high affinity (2.7 nM) for RNA-RNA hybrids. We used the microscale thermo-phoresis method allowing surface independent ...

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Item type:Article
Date:8 June 2017
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.1371/journal.pone.0178875DOI
PONE-D-17-07524Other
Keywords:SOLUTION HYBRIDIZATION ASSAY; TRANSCRIPTIONAL PAUSE SITES; R-LOOP FORMATION; RIBOSOMAL-RNA; STRANDED-RNA; DNA-RNA; HYBRIDS; IMMUNODETECTION; INSTABILITY; TERMINATION;
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:36140
Owner only: item control page

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