Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2

URN to cite this document:: urn:nbn:de:bvb:355-epub-440773
DOI to cite this document:: 10.5283/epub.44077

Penzkofer, Alfons ; Silapetere, Arita ; Hegemann, Peter

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Date of publication of this fulltext: 12 Jan 2021 15:16

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Abstract

Archon2 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense using robotic multidimensional directed evolution approach. Here we report absorption and emission spectroscopic studies of Archon2 in Tris buffer at pH 8. Absorption cross-section spectra, fluorescence quantum distributions, fluorescence quantum yields, and fluorescence excitation spectra were ...