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Nanopore sequencing of RNA and cDNA molecules in Escherichia coli

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Grünberger, Felix ; Ferreira-Cerca, Sébastien ; Grohmann, Dina
License: Creative Commons Attribution Non-commercial 4.0
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Date of publication of this fulltext: 31 Mar 2022 05:15


High-throughput sequencing dramatically changed our view of transcriptome architectures and allowed for ground-breaking discoveries in RNA biology. Recently, sequencing of full-length transcripts based on the single-molecule sequencing platform from Oxford Nanopore Technologies (ONT) was introduced and is widely used to sequence eukaryotic and viral RNAs. However, experimental approaches ...


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