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Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis

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Michl-Holzinger, Philipp ; Obermeyer, Simon ; Markusch, Hanna ; Pfab, Alexander ; Ettner, Andreas ; Bruckmann, Astrid ; Babl, Sabrina ; Längst, Gernot ; Schwartz, Uwe ; Tvardovskiy, Andrey ; Jensen, Ole N. ; Osakabe, Akihisa ; Berger, Frédéric ; Grasser, Klaus D.
Date of publication of this fulltext: 17 Aug 2022 09:12


The heterodimeric histone chaperone FACT, consisting of SSRP1 and SPT16, contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search of post-translational modifications that may regulate the activity of FACT, SSRP1 and SPT16 were isolated from Arabidopsis cells and analysed by mass spectrometry. Four acetylated lysine residues could ...


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