Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

URN to cite this document:: urn:nbn:de:bvb:355-epub-532184
DOI to cite this document:: 10.5283/epub.53218

Wörle, Elisabeth ; Newman, Anthony ; D’Silva, Jovita ; Burgio, Gaetan

; Grohmann, Dina

License: Creative Commons Attribution Non-commercial 4.0
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Date of publication of this fulltext: 17 Nov 2022 15:05

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Abstract

Nucleases derived from the prokaryotic defense system CRISPR-Cas are frequently re-purposed for gene editing and molecular diagnostics. Hence, an in-depth understanding of the molecular mechanisms of these enzymes is of crucial importance. We focused on Cas12a from Francisella novicida (FnCas12a) and investigated the functional role of helix 1, a structural element that together with the bridge ...