nucMACC: An MNase-seq pipeline to identify structurally altered nucleosomes in the genome

URN to cite this document:: urn:nbn:de:bvb:355-epub-586702
DOI to cite this document:: 10.5283/epub.58670

Wernig-Zorc, Sara

; Kugler, Fabian

; Schmutterer, Leo

; Räß, Patrick

; Hausmann, Clemens ; Holzinger, Simon

; Längst, Gernot

; Schwartz, Uwe

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 30 Jul 2024 09:05

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Abstract

Micrococcal nuclease sequencing is the state-of-the-art method for determining chromatin structure and nucleosome positioning. Data analysis is complex due to the AT-dependent sequence bias of the endonuclease and the requirement for high sequencing depth. Here, we present the nucleosome-based MNase accessibility (nucMACC) pipeline unveiling the regulatory chromatin landscape by measuring ...