Calnuc, an EF-Hand Ca 2+ binding protein, specifically interacts with the C-terminal α5-helix of Gαi3

URN to cite this document:: urn:nbn:de:bvb:355-epub-590007
DOI to cite this document:: 10.5283/epub.59000

Lin, Ping ; Fischer, Thierry ; Weiss, Thomas S.

; Farquhar, Marilyn Gist

License: Creative Commons Attribution Non-commercial No Derivatives 4.0
PDF - Published Version
(407kB)

Date of publication of this fulltext: 26 Aug 2024 09:55

Alternative links to fulltext:DOI DOI Publisher

Details

Preview

Export bibliographical data

Abstract

Abstract Calnuc (nucleobindin) was previously shown to be present both in the cytosol and in the Golgi and to be the major Golgi Ca2+ binding protein. In this study we verified the existence of the cytosolic pool of calnuc and investigated its interaction with Gαi3. Cytosolic calnuc was released by mild digitonin permeabilization. In pulse–chase experiments, the two pools of calnuc had different ...