| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Therapeutic Drug Monitoring | ||||
| Publisher: | LIPPINCOTT WILLIAMS & WILKINS | ||||
| Place of Publication: | PHILADELPHIA | ||||
| Volume: | 34 | ||||
| Number of Issue or Book Chapter: | 6 | ||||
| Page Range: | pp. 702-712 | ||||
| Date: | 2012 | ||||
| Institutions: | Medicine > Lehrstuhl für Psychiatrie und Psychotherapie Languages and Literatures > Institut für Information und Medien, Sprache und Kultur (I:IMSK) > Lehrstuhl für Informationswissenschaft (Prof. Dr. Udo Kruschwitz) Informatics and Data Science > Department Human-Centered Computing > Lehrstuhl für Informationswissenschaft (Prof. Dr. Udo Kruschwitz) | ||||
| Identification Number: |
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| Keywords: | LIQUID-CHROMATOGRAPHIC ASSAY; CONSENSUS GUIDELINES; AMANTADINE; PLASMA; PHARMACOTHERAPY; therapeutic drug monitoring; memantine; HPLC/UV; dose-related reference range; individualized dosage | ||||
| Dewey Decimal Classification: | 000 Computer science, information & general works > 020 Library & information sciences 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 63108 |
Web of ScienceAbstract
Background: In the near future, there will be no innovative drug principle for the treatment of dementia. Therefore, optimizing the efficacy of a drug is at present the most promising way to exploit its full pharmacological potential. Method: A high performance liquid chromatography with ultraviolet assay for memantine in serum from demented patients has been developed and validated. The ...
Abstract
Background: In the near future, there will be no innovative drug principle for the treatment of dementia. Therefore, optimizing the efficacy of a drug is at present the most promising way to exploit its full pharmacological potential. Method: A high performance liquid chromatography with ultraviolet assay for memantine in serum from demented patients has been developed and validated. The analytical procedure involves offline solid phase extraction cartridges. Because memantine molecules lack chromophoric pi-electrons, a derivatization with dansyl chloride was required for detection by ultraviolet (UV) photometry. Analyses were performed on a Dionex high-performance liquid chromatography system with a Phenomenex Luna Phenyl-Hexyl analytical column and 0.02 mol/L potassium dihydrogen phosphate buffer/acetonitrile (40/60 V/V) as mobile phase at a flow rate of 0.4 mL/min. Dansylated memantine was detected at 218 nm; 2 more ultraviolet wavelengths at 254 nm and 336 nm were used as an overlay-control check. Results: The retention time for dansylated memantine was 17.1 +/- 0.2 minutes. The calibration curve was linear over a concentration range from 5 to 160 ng/mL (n = 8/r(2) > 0.999). The method had an accuracy of >90%. Intra-assay and inter-assay coefficients of variation were <5% and <13%, respectively, at 3 different concentrations. The limit of quantification and the limit of detection were 2.9 ng/mL and 0.8 ng/mL, respectively. Among 100 substances prescribed as comedications in the treatment of dementia only fluvoxamine and zuclopenthixole showed retention times close to dansylated memantine (17.8 minutes and 18.1 minutes, respectively). However, these 2 drugs were removed from patients' specimens during solid-phase extraction sample preparation. Conclusions: The method is applicable under conditions of daily routine as has been demonstrated by application of the method to patient serum samples. The quantification of 29 samples showed that memantine concentrations suggested as "therapeutic" in the literature may only be reached by high doses of memantine.
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