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Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Agam, Ganesh ; Gebhardt, Christian ; Popara, Milana ; Mächtel, Rebecca ; Folz, Julian ; Ambrose, Benjamin ; Chamachi, Neharika ; Chung, Sang Yoon ; Craggs, Timothy D. ; de Boer, Marijn ; Grohmann, Dina ; Ha, Taekjip ; Hartmann, Andreas ; Hendrix, Jelle ; Hirschfeld, Verena ; Hübner, Christian G. ; Hugel, Thorsten ; Kammerer, Dominik ; Kang, Hyun-Seo ; Kapanidis, Achillefs N. ; Krainer, Georg ; Kramm, Kevin ; Lemke, Edward A. ; Lerner, Eitan ; Margeat, Emmanuel ; Martens, Kirsten ; Michaelis, Jens ; Mitra, Jaba ; Moya Muñoz, Gabriel G. ; Quast, Robert B. ; Robb, Nicole C. ; Sattler, Michael ; Schlierf, Michael ; Schneider, Jonathan ; Schröder, Tim ; Sefer, Anna ; Tan, Piau Siong ; Thurn, Johann ; Tinnefeld, Philip ; van Noort, John ; Weiss, Shimon ; Wendler, Nicolas ; Zijlstra, Niels ; Barth, Anders ; Seidel, Claus A. M. ; Lamb, Don C. ; Cordes, Thorben



Abstract

Single-molecule Forster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of ...

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