Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs

URN to cite this document:: urn:nbn:de:bvb:355-epub-768391
DOI to cite this document:: 10.5283/epub.76839

Latini, Claudia ; Eichlinger, Julian ; Fuchs, Anna-Lisa ; Zhai, Si-Nan ; Ho-Xuan, Hung ; Lehmann, Gerhard ; Glažar, Petar ; Rajewsky, Nikolaus ; Bruckmann, Astrid

; Yang, Li ; Sprangers, Remco

; Meister, Gunter

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 10 Jun 2025 08:13

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Abstract

The ribonuclease DIS3 interacts through its PIN domain with the nuclear exosome and degrades linear RNA substrates using its exoribonuclease domain. However, the PIN domain is also an active endoribonuclease, but cellular substrates are largely unknown. Here, we use a biochemical strategy to find ribonucleases that could degrade circular RNAs (circRNAs). Due to the lack of accessible ends, ...