Woenckhaus, Matthias and Grepmeier, Ulrike and Werner, Bernhard and Schulz, Christian and Rockmann, Felix and Wild, Peter J. and Röckelein, Georg and Blaszyk, Hagen and Schuierer, Marion and Hofstaedter, Ferdinand and Hartmann, Arndt and Dietmaier, Wolfgang (2005) Microsatellite analysis of pleural supernatants could increase sensitivity of pleural fluid cytology. The Journal of molecular diagnostics: JMD 7 (4), pp. 517-524.
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Pleural effusions may result from various inflammatory, hemodynamic, or neoplastic conditions. A common diagnostic problem lies in distinguishing malignant from benign pleural effusions using routine cytological evaluation. We studied pleural fluid samples obtained from 14 patients with histologically confirmed malignancy and from 6 patients with benign pleural effusions using 12 microsatellite markers from 8 different chromosomal regions. Supernatants and cellular sediments of all 20 pleural fluid samples were analyzed. Routine cytological examination was 100% specific for malignancy but was only 57% sensitive. Microsatellite analyses of pleural fluid supernatants showed genetic alterations in tumor patients only. However, 50% of pleural effusions that were considered negative for malignancy by routine cytological analysis showed either loss of heterozygosity or microsatellite instability. The sensitivity of pleural fluid examination rose to 79% when routine cytological assessment was supplemented by molecular studies. Our data suggest that microsatellite analysis increases the sensitivity of cytological pleural fluid examination in assessing potential malignancy and that combining cytological and molecular methods may improve yield and certainty in diagnostically challenging cases.
|Institutions:||Medicine > Lehrstuhl für Pathologie|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||22 Jun 2010 09:57|
|Last Modified:||22 Jun 2010 09:57|