Blesch, A. and Bosserhoff, Anja-Katrin and Apfel, R. and Behl, C. and Hessdoerfer, B. and Schmitt, A. and Jachimczak, P. and Lottspeich, F. and Buettner, R. and Bogdahn, Ulrich
Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA.
Cancer Research 54 (21), pp. 5695-5701.
Growth and progression of malignant melanoma cells is influenced by a complex network of growth-stimulating and -inhibiting factors produced by both the tumor cells and the local environment. Here we report the purification and molecular cloning of a novel growth regulating protein, designated melanoma inhibitory activity (MIA) and provide a preliminary functional characterization. MIA is translated as a 131-amino acid precursor and processed into a mature 107-amino acid protein after cleavage of a putative secretion signal. A murine complementary DNA was isolated that encoded a MIA-protein with 88% amino acid identity. MIA is secreted into the culture supernatant by several malignant melanoma cell lines as an M(r) 11,000 autocrine growth factor and acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas. MIA has no homology to any other known protein and, therefore, represents a novel type of growth-regulatory factor. Furthermore, we describe a molecular approach to express functionally active MIA in Escherichia coli, which might be attractive as a future antitumor therapeutical substance.
|Institutions:|| Medicine > Lehrstuhl für Neurologie > Arbeitsgruppe NeuroOnkologie|
Medicine > Lehrstuhl für Pathologie
|Amino Acid Sequence||MESH|
|Extracellular Matrix Proteins||MESH|
|Molecular Sequence Data||MESH|
|Neoplasm Proteins/isolation & purification||MESH|
|Recombinant Proteins/isolation & purification||MESH|
|Tumor Cells, Cultured||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||07 Jun 2011 06:22|
|Last Modified:||23 Jan 2014 10:58|