Köppl, Christine and Gleich, Otto (1988) Cobalt labelling of single primary auditory neurones: An alternative to HRP. Hearing Research 32 (2), pp. 111-116.
We have labelled single, primary auditory neurones in three reptile and one bird species. After functional characterization of the neurones, hexamminecobaltic chloride was iontophoretically injected through the recording micropipette. Precipitation of cobalt sulfide followed by silver intensification of the cochlear duct as a whole-mount preparation revealed stained neurones in over 90% of cases. This method has several advantages over labelling with HRP.
|Institutions:||Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde|
|Keywords:||Neurone labelling; Auditory nerve; Cobalt labelling; Lizard; Bird; Histological techniques|
|Subjects:||500 Science > 590 Zoological sciences|
600 Technology > 610 Medical sciences Medicine
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||No|
|Deposited On:||27 May 2008 14:11|
|Last Modified:||20 Jul 2011 21:15|