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Time-resolved quantitative assays and imaging of enzymes and enzyme substrates using a new europium fluorescent probe for hydrogen peroxide

URN to cite this document:
urn:nbn:de:bvb:355-opus-3326
DOI to cite this document:
10.5283/epub.10248
Wu, Meng
Date of publication of this fulltext: 02 Feb 2005 06:48


Abstract (English)

The thesis describes the development of a novel reversible H2O2 fluorescent probe comprising the ternary complex of europium(III), tetracycline and hydrogen peroxide; and its application in the assays of H2O2, of H2O2 producing oxidases and their substrates, and of H2O2 consuming catalase and its inhibitors. The probe is applied in steady-state intensity-based, time-resolved �gated�, or ...

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Translation of the abstract (German)

Diese Arbeit beschreibt die Entwicklung einer neuen reversiblen Fluoreszenzsonde für H2O2, ein ternärer Komplex, bestehend aus Europium(III), Tetracyclin und Wasserstoffperoxid, und seine Anwendungen in Untersuchungen von H2O2, H2O2-produzierenden Oxidasen und ihren Substraten, von H2O2-verbrauchender Katalase und dessen Inhibitoren. Die Sonde wurde durch stationäre intensitätsbasierende, ...

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