A flow cytometric screening test for detergent-resistant surface antigens in monocytes

DOI to cite this document:: 10.5283/epub.1028

Wolf, Zsuzsanna ; Orsó, Evelyn ; Werner, Tobias ; Boettcher, Alfred ; Schmitz, Gerd

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Date of publication of this fulltext: 05 Aug 2009 13:25

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Abstract

Rafts resemble cholesterol- and glycosphingolipid-enriched, liquid-ordered plasma membrane microdomains, showing resistance to nonionic detergents, and are involved in various cellular processes. In the present study, we have tested surface antigens on resting and lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes for their detergent resistance (i.e. raft-association), by flow ...