Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages

DOI to cite this document:: 10.5283/epub.1037

Werner, Tobias ; Liebisch, Gerhard ; Grandl, Margot ; Schmitz, Gerd

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Date of publication of this fulltext: 05 Aug 2009 13:25

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Abstract

BACKGROUND: For understanding cholesterol and phospholipid efflux pathways there is a need for cellular fluorescence-based high-content screens (HCS) to investigated the cholesterol and phospholipid content in human macrophages. METHODS: Making use of fluorescence imaging based on HCS we have developed a tool to evaluate new agents that can act as inducers of cholesterol efflux. The fluorescence ...