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A spin labeled and highly resolved ed-H(CCO)NH-TOCSY experiment for the facilitated assignment of proton side chains in partially deuterated samples

Gschwind, R. M. ; Gemmecker, G. ; Kessler, H.


The introduction of deuterated and partially deuterated protein samples has greatly facilitated the 13C assignment of larger proteins. Here we present a new version of the HC(CO)NH-TOCSY experiment, the ed-H(CCO)NH-TOCSY experiment for partially deuterated samples, introducing a multi-quantum proton evolution period. This approach removes the main relaxation source (the dipolar coupling to the ...


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