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Studies on the NusB protein of Escherichia coli; Expression and determination of the secondary structure elements by multinuclear NMR spectroscopy

Berglechner, F. ; Richter, G. ; Fischer, M. ; Bacher, A. ; Gschwind, R. M. ; Huenges, M. ; Gemmecker, G. ; Kessler, H.


The product of the nusB gene of Escherichia coli modulates the efficiency of transcription termination at I Z M ~(N utilization) sites of various bacterial and bacteriophage i genes. Similar control mechanisms operate in eukaryotic viruses (e.g. human immunodeficiency virus). A recombinant strain of E. coli producing relatively large amounts of NusB protein (about 10% of cell protein) was ...


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