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Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate

Hecht, S., Kis, F., Eisenreich, W., Amslinger, S., Wungsintaweekul, J., Herz, S., Rohdich, F. and Bacher, A. (2001) Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate. J. Org. Chem. 66 (11), pp. 3948-3952.

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Recombinant l-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/ or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of l-deoxy-rt-xylulose 5-phosphate carrying C-13 Or C-14 from commercially available precursors with an overall yield around 50%.

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Item type:Article
Additional Information (public):Times Cited: 16
Institutions:Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger
Identification Number:
ISI:000168911300039Web of Science ID
Keywords:Escherichia-coli biosynthesis precursor protein thiamin 4-diphosphocytidyl-2c-methyl-d-erythritol d-1-deoxyxylulose isoprenoids terpenoids catalyzes
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Item ID:11075
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