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Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate

Hecht, S. ; Kis, F. ; Eisenreich, W. ; Amslinger, S. ; Wungsintaweekul, J. ; Herz, S. ; Rohdich, F. ; Bacher, A.


Recombinant l-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/ or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of l-deoxy-rt-xylulose 5-phosphate carrying C-13 Or C-14 from commercially available precursors with an overall yield around 50%.

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