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Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways

Laupitz, R. ; Hecht, S. ; Amslinger, S. ; Zepeck, F. ; Kaiser, J. ; Richter, G. ; Schramek, N. ; Steinbacher, S. ; Huber, R. ; Arigoni, D. ; Bacher, A. ; Eisenreich, W. ; Rohdich, F.



Abstract

An open reading frame (Acc. no. P50740) on the Bacillus subtilis chromosome extending from bp 184 997-186 043 with similarity to the idi-2 gene of Streptomyces sp. CL190 specifying type II isopentenyl diphosphate isomerase was expressed in a recombinant Escherichia coli strain. The recombinant protein with a subunit mass of 39 kDa was purified to apparent homogeneity by column chromatography. The ...

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