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Projected structure of the surface protein of Deinococcus radiodurans determined to 8 Å resolution by cryomicroscopy

Rachel, Reinhard ; Jakubowski, U. ; Tietz, H. ; Hegerl, R. ; Baumeister, W.


Cryomicroscopy at liquid nitrogen temperature has been used, in conjunction with correlation averaging, to determine the projection structure of the surface protein (HPI layer) of the Bacterium Deinococcus radiodurans to a resolution of 0.83 nm. The two-dimensional crystals were embedded in a thin layer of frozen-hydrated aurothioglucose, obtained by the controlled evaporation of an ...


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