Item type: | Article | ||||||||||||||||||||
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Journal or Publication Title: | Archives of microbiology | ||||||||||||||||||||
Publisher: | SPRINGER | ||||||||||||||||||||
Place of Publication: | NEW YORK | ||||||||||||||||||||
Volume: | 190 | ||||||||||||||||||||
Number of Issue or Book Chapter: | 3 | ||||||||||||||||||||
Page Range: | pp. 379-394 | ||||||||||||||||||||
Date: | 2008 | ||||||||||||||||||||
Institutions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel | ||||||||||||||||||||
Identification Number: |
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Classification: |
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Keywords: | NANOARCHAEUM-EQUITANS; CRYSTAL-STRUCTURE; OUTER-MEMBRANE; MECHANOSENSITIVE CHANNEL; HALOBACTERIUM-SALINARUM; ARCHAEON IGNICOCCUS; PYRODICTIUM-ABYSSI; SP-NOV; COMPLEX; ARCHAEBACTERIUM; Crenarchaeota; chemolithoautotrophy; proteome; MALDI; Ignicoccus hospitalis | ||||||||||||||||||||
Dewey Decimal Classification: | 500 Science > 570 Life sciences | ||||||||||||||||||||
Status: | Published | ||||||||||||||||||||
Refereed: | Yes, this version has been refereed | ||||||||||||||||||||
Created at the University of Regensburg: | Yes | ||||||||||||||||||||
Item ID: | 13220 |
Abstract
Ignicoccus hospitalis, a hyperthermophilic, chemolithoautotrophic Crenarchaeon, is the host of Nanoarchaeum equitans. Together, they form an intimate association, the first among Archaea. Membranes are of fundamental importance for the interaction of I. hospitalis and N. equitans, as they harbour the proteins necessary for the transport of macromolecules like lipids, amino acids, and cofactors ...

Abstract
Ignicoccus hospitalis, a hyperthermophilic, chemolithoautotrophic Crenarchaeon, is the host of Nanoarchaeum equitans. Together, they form an intimate association, the first among Archaea. Membranes are of fundamental importance for the interaction of I. hospitalis and N. equitans, as they harbour the proteins necessary for the transport of macromolecules like lipids, amino acids, and cofactors between these organisms. Here, we investigated the protein inventory of I. hospitalis cells, and were able to identify 20 proteins in total. Experimental evidence and predictions let us conclude that 11 are soluble cytosolic proteins, eight membrane or membrane-associated proteins, and a single one extracellular. The quantitatively dominating proteins in the cytoplasm (peroxiredoxin; thermosome) antagonize oxidative and temperature stress which I. hospitalis cells are exposed to at optimal growth conditions. Three abundant membrane protein complexes are found: the major protein of the outer membrane, which might protect the cell against the hostile environment, forms oligomeric complexes with pores of unknown selectivity; two other complexes of the cytoplasmic membrane, the hydrogenase and the ATP synthase, play a key role in energy production and conversion.
Metadata last modified: 29 Sep 2021 07:31