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Electron tomography of ice-embedded prokaryotic cells

Grimm, R. ; Singh, H. ; Rachel, Reinhard ; Typke, D. ; Zillig, W. ; Baumeister, W.


Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a resolution of 20-40 nm from tilt series comprising 50-140 images. The dose was kept within tolerable limits. A resolution of 20 nm allowed ...


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