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Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins

Hagen, Ilja ; Ecker, Margit ; Lagorce, Arnaud ; Francois, Jean M. ; Sestak, Sergej ; Rachel, Reinhard ; Grossmann, Guido ; Hauser, Nicole C. ; Hoheisel, Jörg D. ; Tanner, Widmar ; Strahl, Sabine



Abstract

The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of > 300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and ...

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