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The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre

Urich, Tim ; Bandeiras, Tiago M. ; Leal, Sónia S. ; Rachel, Reinhard ; Albrecht, Till ; Zimmermann, Peter ; Scholz, Corinna ; Teixeira, Miguel ; Gomes, Cláudio M. ; Kletzin, Arnulf



Abstract

The SOR (sulphur oxygenase reductase) is the initial enzyme in the sulphur-oxidation pathway of Acidianus ambivalens. Expression of the sor gene in Escherichia coli resulted in active, soluble SOR and in inclusion bodies from which active SOR could be refolded as long as ferric ions were present in the refolding solution. Wild-type, recombinant and refolded SOR possessed indistinguishable ...

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