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Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression

Veith, Andreas ; Klingl, Andreas ; Zolghadr, Behnam ; Lauber, Karin ; Mentele, Reinhard ; Lottspeich, Friedrich ; Rachel, Reinhard ; Albers, Sonja-Verena ; Kletzin, Arnulf



Abstract

The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the chromosomes, are constitutively transcribed as bicistronic operons in A. ...

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