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Cytotoxic effector cell function at different stages of human monocyte-macrophage maturation

Andreesen, Reinhard ; Osterholz, J. ; Bross, K. J. ; Schulz, A. ; Luckenbach, G. A. ; Löhr, G. W.


Human blood-borne monocytes were cultured for up to 22 days on disposable Teflon foils. Within 8 days, these monocytes developed into mature macrophages. At various stages of differentiation, the cells were recovered from the hydrophobic membrane and were assayed for typical monocyte-macrophage enzymes and morphology, binding of monoclonal antibodies (OKM1, OKla1), Fc and transferrin receptors, ...


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