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Human macrophage maturation in vitro: expression of functional transferrin binding sites of high affinity

Andreesen, Reinhard ; Sephton, R. G. ; Gadd, S. ; Atkins, R. C. ; De Abrew, S.



Abstract

Human blood monocytes (mo) when cultured in suspension on hydrophobic teflon membranes undergo terminal maturation to macrophages (MO). Together with the appearance of new lineage-restricted differentiation antigens, mature MO but not blood mo, express transferrin (TF) receptor molecules as detected by immunostaining methods. Here we report that radio- and fluorescein-labelled TF binds to a ...

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