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Microculture assay for human macrophage maturation in vitro. Cell-ELISA analysis of differentiation antigen expression

Andreesen, Reinhard ; Mackensen, A. ; Osterholz, J. ; Brugger, W. ; Löhr, G. W.


The terminal differentiation of monocytes (mo) to macrophages (MO) is essential for the complex functioning of the mononuclear phagocyte system. Similar to in vivo MO maturation, blood mo transform to MO when cultured in vitro. This offers a model system for experimental studies of functional defects within this cell lineage. In order to develop a microassay for the evaluation of MO maturation in ...


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