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Phagocytosis of nanoparticles by human immunodeficiency virus (HIV)-infected macrophages: a possibility for antiviral drug targeting

Schäfer, V. ; von Briesen, H. ; Andreesen, Reinhard ; Steffan, A. M. ; Royer, C. ; Tröster, S. ; Kreuter, J. ; Rübsamen-Waigmann, H.



Abstract

Human monocytes/macrophages (MO/MAC) were isolated from peripheral blood and cultivated on hydrophobic Teflon membranes. This culture system is suitable for HIV infection of MO/MAC in vitro. After transfer into 24-well plates the mature macrophages (infected or uninfected) were used for measurements of phagocytosis. The uptake of different, radioactively labeled nanoparticles (NP) made of ...

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