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Allostimulatory cells in fresh human blood: heterogeneity in antigen-presenting cell populations

Egner, W. ; Andreesen, Reinhard ; Hart, D. N.


The relative ability of unmanipulated monocytes, B cells, and dendritic cells (DC) from peripheral blood to stimulate an allogeneic MLR has not been clearly established. We studied the allostimulatory ability of these cell types from minimally manipulated PBMC populations to exclude the induction of stimulatory properties by the complex isolation procedures commonly used to isolate blood DC. ...


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