Schlesier, M., Krause, S., Dräger, R., Wolff-Vorbeck, G., Kreutz, Marina, Andreesen, Reinhard and Peter, H. H.
(1994)
Monocyte differentiation and accessory function: different effects on the proliferative responses of an autoreactive T cell clone as compared to alloreactive or antigen-specific T cell lines and primary mixed lymphocyte cultures.
Immunobiology 190 (1-2), pp. 164-174.
Date of publication of this fulltext: 14 Apr 2010 05:10at PubMed
Abstract
An autoreactive T cell clone derived from a patient with reactive arthritis, two alloreactive T cell lines, two antigen-specific T cell lines and allogeneic resting T cells were analyzed for their responses to monocytes and macrophages derived from monocytes by in vitro differentiation. The autoreactive T cell clone strongly proliferated in response to fresh monocytes and to macrophages derived ...
Abstract
An autoreactive T cell clone derived from a patient with reactive arthritis, two alloreactive T cell lines, two antigen-specific T cell lines and allogeneic resting T cells were analyzed for their responses to monocytes and macrophages derived from monocytes by in vitro differentiation. The autoreactive T cell clone strongly proliferated in response to fresh monocytes and to macrophages derived from a 7 day culture, but only poorly to monocytes cultured for 2 days. In contrast, alloreactive and antigen-specific T cell lines proliferated to all stimulatory cells equally well. Finally, primary mixed lymphocyte reactions could be stimulated by both fresh and 2-day cultured monocytes, but not by in vitro derived macrophages. The impaired response of the autoreactive T cell clone to 2-day cultured monocytes could not be attributed to reduced expression of several well-defined surface molecules nor to induction of nonresponsiveness. Neither allogeneic monocytes nor cytokines (IL-1, IL-2, IL-4, IL-6) could correct the defective response of the autoreactive T cell clone. However, preculture of monocytes in the presence of interferon-gamma, IL-1, IL-4 or IL-6 retained their stimulatory capacity. Our interpretation of the selectively impaired response of the autoreactive T cell clone is that it most likely recognizes a differentiation-dependent monocyte/macrophage-specific peptide.
Export bibliographical data
| Item type: | Article |
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| Date: | 1994 |
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| Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
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| Identification Number: | | Value | Type |
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| 7521857 | PubMed ID |
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| Classification: | | Notation | Type |
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| Antigen-Presenting Cells/immunology | MESH | | Antigens, Surface/biosynthesis | MESH | | Autoantigens/immunology | MESH | | Cell Differentiation/physiology | MESH | | Cell Division/immunology | MESH | | Cell Line | MESH | | Epitopes/immunology | MESH | | Humans | MESH | | Isoantigens/immunology | MESH | | Lymphocyte Activation/immunology | MESH | | Lymphocyte Culture Test, Mixed | MESH | | Monocytes/immunology | MESH | | T-Lymphocyte Subsets/immunology | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Yes |
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| Item ID: | 14286 |
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