Monocyte differentiation and accessory function: different effects on the proliferative responses of an autoreactive T cell clone as compared to alloreactive or antigen-specific T cell lines and primary mixed lymphocyte cultures

URN to cite this document:

urn:nbn:de:bvb:355-epub-142861 Copy

DOI to cite this document:

10.5283/epub.14286 Copy

Schlesier, M. ; Krause, S. ; Dräger, R. ; Wolff-Vorbeck, G. ; Kreutz, Marina ; Andreesen, Reinhard ; Peter, H. H.

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Date of publication of this fulltext: 14 Apr 2010 05:10

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Details

Item type:	Article
Journal or Publication Title:	Immunobiology
Publisher:	Elsevier
Volume:	190
Number of Issue or Book Chapter:	1-2
Page Range:	pp. 164-174
Date:	1994
Institutions:	Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Identification Number:	Value Type 7521857 PubMed ID
Classification:	Notation Type Antigen-Presenting Cells/immunology MESH Antigens, Surface/biosynthesis MESH Autoantigens/immunology MESH Cell Differentiation/physiology MESH Cell Division/immunology MESH Cell Line MESH Epitopes/immunology MESH Humans MESH Isoantigens/immunology MESH Lymphocyte Activation/immunology MESH Lymphocyte Culture Test, Mixed MESH Monocytes/immunology MESH T-Lymphocyte Subsets/immunology MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	14286

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Abstract

An autoreactive T cell clone derived from a patient with reactive arthritis, two alloreactive T cell lines, two antigen-specific T cell lines and allogeneic resting T cells were analyzed for their responses to monocytes and macrophages derived from monocytes by in vitro differentiation. The autoreactive T cell clone strongly proliferated in response to fresh monocytes and to macrophages derived ...

Abstract

An autoreactive T cell clone derived from a patient with reactive arthritis, two alloreactive T cell lines, two antigen-specific T cell lines and allogeneic resting T cells were analyzed for their responses to monocytes and macrophages derived from monocytes by in vitro differentiation. The autoreactive T cell clone strongly proliferated in response to fresh monocytes and to macrophages derived from a 7 day culture, but only poorly to monocytes cultured for 2 days. In contrast, alloreactive and antigen-specific T cell lines proliferated to all stimulatory cells equally well. Finally, primary mixed lymphocyte reactions could be stimulated by both fresh and 2-day cultured monocytes, but not by in vitro derived macrophages. The impaired response of the autoreactive T cell clone to 2-day cultured monocytes could not be attributed to reduced expression of several well-defined surface molecules nor to induction of nonresponsiveness. Neither allogeneic monocytes nor cytokines (IL-1, IL-2, IL-4, IL-6) could correct the defective response of the autoreactive T cell clone. However, preculture of monocytes in the presence of interferon-gamma, IL-1, IL-4 or IL-6 retained their stimulatory capacity. Our interpretation of the selectively impaired response of the autoreactive T cell clone is that it most likely recognizes a differentiation-dependent monocyte/macrophage-specific peptide.

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