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Isolation and phenotypic characterization of colonic macrophages

Rogler, G. ; Hausmann, M. ; Vogl, D. ; Aschenbrenner, E. ; Andus, T. ; Falk, W. ; Andreesen, Reinhard ; Schölmerich, J. ; Gross, V.



Abstract

Macrophages play an important role in the intestinal mucosal immune system. However, they are a poorly defined cell population. We therefore determined their phenotype in normal colonic mucosa. Macrophages were isolated from colonic biopsies and surgical specimens by collagenase digestion. Colonic macrophages were positively sorted by anti-CD33 magnetic beads. Flow cytometric triple fluorescence ...

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