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Monocyte differentiation in intestine-like macrophage phenotype induced by epithelial cells

Spöttl, T. ; Hausmann, M. ; Kreutz, M. ; Peuker, A. ; Vogl, D. ; Schölmerich, J. ; Falkenstein, W. ; Andreesen, Reinhard ; Andus, T. ; Herfarth, H. ; Rogler, G.


Macrophages in normal colonic mucosa show a specific and distinct phenotype with low expression of the typical monocyte/macrophage surface antigens CD14, CD16, and CD11b and T-cell costimulatory molecules. A method for the in vitro induction of a macrophage phenotype similar to this intestinal phenotype is presented. Multicellular spheroids (MCSs) of intestinal epithelial cell (IEC) and control ...


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